Insertion Element IS 3 -Based PCR Method for Subtyping Escherichia coli O157:H7
نویسندگان
چکیده
منابع مشابه
Transposition of cyanobacterium insertion element ISY100 in Escherichia coli.
The genome of the cyanobacterium Synechocystis sp. strain PCC6803 has nine kinds of insertion sequence (IS) elements, of which ISY100 in 22 copies is the most abundant. A typical ISY100 member is 947 bp long and has imperfect terminal inverted repeat sequences. It has an open reading frame encoding a 282-amino-acid protein that appears to have partial homology with the transposase encoded by a ...
متن کاملSelenocysteine insertion directed by the 3′-UTR SECIS element in Escherichia coli
Co-translational insertion of selenocysteine (Sec) into proteins in response to UGA codons is directed by selenocysteine insertion sequence (SECIS) elements. In known bacterial selenoprotein genes, SECIS elements are located in the coding regions immediately downstream of UGA codons. Here, we report that a distant SECIS element can also function in Sec insertion in bacteria provided that it is ...
متن کاملInsertion element IS121 is near proA in the chromosomes of Escherichia coli K-12 strains.
Insertion element IS121 was mapped between proA and a previously mapped IS5A element in two F-prime plasmids. Results of hybridizations of IS121 to chromosomal DNA from four other strains suggest that IS121 is normally present at this position in the chromosomes of Escherichia coli K-12 strains.
متن کاملDevelopment of a rapid PCR method using the insertion sequence IS1203 for genotyping Shiga toxin-producing Escherichia coli O157.
We developed a rapid PCR method utilizing the diversity of the insertion site IS1203 for genotyping Shiga toxin-producing Escherichia coli (STEC) O157 (IS1203 PCR typing). DNA fragments digested by PvuII, which cut IS1203 at one site, were ligated with themselves and detected by PCR with outward-facing primer pairs for IS1203. To minimize nonspecific bands, nested PCR was also performed. Two fi...
متن کاملDevelopment of a multiplex PCR-based rapid typing method for enterohemorrhagic Escherichia coli O157 strains.
Enterohemorrhagic Escherichia coli O157 (EHEC O157) is a food-borne pathogen that has raised worldwide public health concern. The development of simple and rapid strain-typing methods is crucial for the rapid detection and surveillance of EHEC O157 outbreaks. In the present study, we developed a multiplex PCR-based strain-typing method for EHEC O157, which is based on the variability in genomic...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1998
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.36.5.1180-1184.1998